Composite

Part:BBa_K618111:Design

Designed by: Chris Bate, Ben Park, Marc Ammerlaan   Group: iGEM11_Michigan   (2011-09-27)

pBAD ompA-Gli1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


Design Notes

NOTE- the sequence as listed here is wrong. Because part K165007 is supplied on a Silver lab vector (Bba_J63009 or V0002) with a modified biobrick prefix, the scar between ompA and Gli-1 is non-standard. The G at position 840 is not there. This has been verified by sequencing. Deleting the G keeps Gli-1 in frame with ompA. Part length is 1364bp.

Sequencing also revealed that one base in the scar between pBAD and ompA seems to have mutated (#361 T->A). This is true for both constructs (K618111 & K618112) utilizing pBAD. Possibly this means that the mutation exists in the suffix of part K259007 as supplied this year. This change should have no impact on the protein as it occurs between the RBS and start codon.

The sequence was generated automatically and I couldn't figure out how to edit it.

The link below contains the (AFAIK) correct sequence in genbank format:

Media:K618111.gb

Source

parts come from iGEM distribution and from iGEM HQ

References